Generator

Part:BBa_K090002:Design

Designed by: Ellis O'Neill   Group: iGEM08_Cambridge   (2008-10-23)

Constitutively expresses glutamate gated K+ channel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo. Promoter is constitutive, medium strength. RBS is medium strength.There is a BamHI site either side of the promoter to allow easy removal. There is also no scar sites as the entire construct was synthesised in it's entirety. Terminator is very strong artificial.



Source

From genome of Synechocystis PCC 6803.

References