Generator
Part:BBa_K090002:Design
Designed by: Ellis O'Neill Group: iGEM08_Cambridge (2008-10-23)
Constitutively expresses glutamate gated K+ channel
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
Illegal AgeI site found at 484 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo. Promoter is constitutive, medium strength. RBS is medium strength.There is a BamHI site either side of the promoter to allow easy removal. There is also no scar sites as the entire construct was synthesised in it's entirety. Terminator is very strong artificial.
Source
From genome of Synechocystis PCC 6803.